A Study to Evaluate Sampling Methods for Subgingival Plaque. The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.

Subgingivale Plaque, Plaqueentnahme, Plaqueentnahmetechnik, Parodiagnostik, Plaque-Analyse mit Real Time PCR, Papierspitzen, subgingival plaque, plaque sampling techniques, plaque analysis with real-time PCR, paper point sampling, curette sampling

Counts of 40 subgingival spe- cies wore determined in each plaque sample using the checkerboard DNA-DNA hybridization technique (Socran-sky et al. I994). After the removal of supragingival plaque, subgingival Two Subgingival Plaque‐Sampling Strategies Used With RNA Probes. Diana‐Maria Krigar Objective: Comparison of the results of microbiological RNA-probeanalysis after subgingival plaque sampling according to 2 differentstrategies.

Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Of the sampling of all assessed sites, the highest probability for simultaneously detecting the tested pathogens was found in respect to the combination of supra- and subgingival plaque samples 1994-08-01 Conclusion: Salivary analysis might be discussed as a potential alternative to subgingival plaque sampling for microbiologic analysis in both AgP and CP. Discover the world's research. Subgingival plaque sampling and clinical recording (at baseline) and scaling and root planing was performed. Two weeks later the selected periodontal sites were submitted to one of the following treatments: Irrigation with a hydroalcoholic solution of propolis extract twice a week for 2 weeks (group A); irrigation with a placebo twice a week for 2 weeks (group B); or no additional treatment Before and immediately after treatment, subgingival plaque samples were taken from interdental sites with 3 to 5 mm probing depth (PD) at 2 test teeth and 2 positive control teeth. To evaluate the influence of sampling on the microflora, plaque samples were also taken twice at 2 teeth without therapy with PD of 3 to 5 mm (negative control).

Subgingival plaque was collected from a total of 90 pockets across all subjects. Subsequently, subgingival ultrasonic instrumentation (SUI) was performed by using EO or saline as the irrigation agent. After continuous mouth rinsing at home with EO or saline for 7 days, subgingival plaques were sampled again.

the subgingival microbiota, pathogens, and plaque [12]. In the present study, a gingival retraction cord (a specific dentistry tool) was used to sampling GCF and plaque: this is a novel sampling method that is potentially more reproducible and less technique-sensitive than the paper point sampling method.

Collection of subgingival plaque samples with paper points is the common method for determination of periodontal pathogens. 21,24, 25, 27,28 However, sampling subgingival plaque with paper points The full text of this article hosted at iucr.org is unavailable due to technical difficulties.

subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes Bernadette Pretzl, Jule Paul, Diana M. Krigar, Lorenz Uhlmann, Peter Eickholz & Bettina Dannewitz

After supragingival plaque was carefully removed and sampling sites were isolated with cotton rolls and air-dried, a subgingival plaque sample was collected with 2 sterile paper points per site.

Listing a study does not mean it has been evaluated by the U.S. Federal Government. Sampling was always performed after standardized oral hygiene instructions over a period of 3 weeks. Prior to subgingival sampling, supragingival plaque—as disclosed by an indicator—was removed with a previously unused toothbrush and water performed by the children themselves. No toothpaste was used. Methods: In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse.
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Detection of subgingival periodontal pathogens--comparison of two sampling strategies.

Two weeks later irrigation procedures were 7 Feb 2017 The hypervariable region V4 of the 16S rDNA gene was targeted to explore richness and diversity of subgingival plaque samples [11]. Periodontitis is an attachment loss and inflammatory disease mainly caused by bacteria pathogens in subgingival plaque caused by immune-inflammatory periodontal disease; supragingival plaque; supra- and subgingival plaque samples from periodontitis subjects.
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Disadvantages of the technique. require training for proper collection; specific equipment and supplies required for sampling; More validation is needed.

Oral Microbiol Immunol. 1987 Sep;2(3):142-4. Sampling of subgingival plaque: a comparison of two methods using darkfield microscopy. Strand P, Palmer RM, Wilson RF. METHODS: In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse. Analysis was performed using a commercially available polymerase chain reaction test for 11 periodontal pathogens.